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	Provedor de dados BABT (1) Rev. Bras. Fisiol. Veg. (1) Autor BAÚ,SANDRA M. T. (1) Davide,Antonio Claudio (1) José,Anderson Cleiton (1) MAZZAFERA,PAULO (1) SANTORO,LUIZ G. (1) Silva,Edvaldo Aparecido Amaral da (1) Toorop,Peter (1) Mais... Palavra-chave Legumin (2) Coffea (1) Desiccation tolerance (1) Endosperm (1) Forest seed (1) Proteomics (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2011 (1) 2001 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Protein expression upon desiccation and imbibition of Magnolia ovata A. St.-Hil seeds BABT José,Anderson Cleiton; Silva,Edvaldo Aparecido Amaral da; Davide,Antonio Claudio; Toorop,Peter. The effect of seed drying and imbibition was studied by differential protein expression using two dimensional gel electrophoresis. After drying to a range of water contents, seeds were germinated to assess the viability. Seeds of Magnolia ovata did not withstand the desiccation down to 0.10 g H2O · g-1 dw. The critical water content below which the desiccation sensitivity became apparent was around 0.18 g H2O · g-1 dw (-26.5 MPa). Total protein was extracted and separated by 2D electrophoresis from fresh seeds (0.28 g H2O · g-1 dw), mild dried seeds (0.25 g H2O · g-1 dw) and seeds at low water content (0.10 g H2O · g-1 dw) before and after imbibition for 10 days. The proteome profile revealed the presence of 588 spots on each silver stained gel, from which... Tipo: Info:eu-repo/semantics/article Palavras-chave: Desiccation tolerance; Forest seed; Legumin; Proteomics. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000300006 Seed storage proteins in coffee Rev. Bras. Fisiol. Veg. BAÚ,SANDRA M. T.; MAZZAFERA,PAULO; SANTORO,LUIZ G.. It has been reported that Coffea arabica seeds contain as the main reserve protein, a legumin-like protein, constituted of two subunits, alpha and beta, of approximately 35 and 20 kDa. In this work the seed proteins of several coffee species and varieties were investigated by SDS-PAGE and gel filtration. No differences were observed in the electrophoretic profiles among varieties of C. arabica, however, marked differences were observed among species, or even among individuals of some species. In general, the molecular weight of the subunits alpha and beta accounted for a monomer of 48 to 62 kDa. However, native molecular weight obtained by gel filtration showed that for most of the species there is association of 6 of such proteins, in a hexamer. The most... Tipo: Info:eu-repo/semantics/article Palavras-chave: Coffea; Legumin; Endosperm. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-31312001000100004 Registros recuperados: 2 Primeira ... 1 ... Última

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